AN INNOVATIVE METHOD OF DISPERSIVE THREE LIQUID MICROEXTRACTION COMBINED WITH HPLC-UV FOR THE DETERMINATION OF VITAMIN B1 IN SOUR CHERRY JUICE

The present study examined an innovative method for determining the level of vitamin B1 in sour cherry juice. Dispersive three liquid microextraction (DTLME) is a rapid, simple, and sensitive technique. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: a mobile phase of 5% methanol and 95% aqueous phase (1 M KH2PO4 water solution), a flow rate of 1.0 mL min^–1 and a detection wavelength of 260 nm. The extraction efficiency of vitamin B1 was influenced by factors such as sodium chloride, the type and volume of disperser, and the extraction solvents. The limits of detection (LOD) and quantification (LOQ) of the proposed approach were 0.15 and 0.5 ng mL^–1, respectively, for vitamin B1. The relative standard deviation (RSD) for the three replicate determinations at 10 ng mL^–1 was less than 3.63%. Appropriate linear behaviour over the observed concentration range was obtained with a value of R² 0.996 for vitamin B1. This method was successfully applied to sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, is a local variety of sour cherry typically found in the high altitude areas of the Isfahan province in Iran.