Genotypic Characteristics of Clinical and Non-Clinical Isolates of Pseudomonas aeruginosa: Distribution of Different antibiogram Profiles and Molecular Typing

The present study aims to characterize Pseudomonas aeruginosa isolates in three hospitals in Hadhramout, Yemen. Two hundred samples were collected from patients, health workers staff hands and environmental samples and P. aeruginosa isolates were typed using antibiotyping and ERIC-PCR. The results showed that eleven yielded ERIC genetic patterns (PIPXI) and size of amplified DNA bands size approximately ranged from 150-200 bp to 450-500 bp per sample, the highest rate of isolates from genetic patterns (ERIC-PCR) PI and PII was 23.5%. ERIC-PCR typing results showed horizontal transmission from patient to patient who had the same genetic patterns, isolates from patients linked to all isolates from environmental sources and staff hand samples which had the same genetic patterns, that indicated there was direct relationships between them. About 16 Antimicrobial Resistance Profile (ARP) including resistance ranged from 5 to 13 antimicrobial. Resistance pattern A1 (48.2%) isolates was the more frequent. Antibiogram typing showed a link between strains isolated from patients and environmental sources, but failed to show a direct relationships between patients and staff hands samples. ERIC-PCR typing was a more precise molecular technique than antibiogram methods and should be used for monitoring and determination of the sources of infection.