Author/Authors :
Askari, Azam rafsanjan university of medical sciences - Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran, ايران , Hassanshahi, Gholam Hossein rafsanjan university of medical sciences - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran, ايران , Ghalebi, Seyed Razi yazd shahid sadoghi university of medical sciences - Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran, ايران , Jafarzadeh, Abdollah ahvaz jundishapur university of medical sciences - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran, اهواز, ايران , Mohit, Maryam kerman university of medical sciences - Department of Pathology, Kerman University of Medical Sciences, Kerman, IR Iran, ايران , Hajghani, Masomeh rafsanjan university of medical sciences - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran, ايران , Kazemi Arababadi, Mohammad rafsanjan university of medical sciences - Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IR Iran, ايران
Abstract :
Background: Occult hepatitis B infected (OBI) patients cannot eradicate hepatitis B virus (HBV)-DNA from their liver and peripheral blood, completely. Objectives: The main aim of this study was to investigate the rate of HLA-A2 expression on peripheral blood mononuclear cells (PBMCs) of patients with OBI. Materials and Methods: In this experimental study, intensity of HLA-A2 was measured on the PBMCs of 57 OBI patients and 100 HBsAg-/anti-HBc+/HBV-DNA samples were enrolled as controls; measurements were performed using the flow cytometry technique. Results: Flow cytometric analysis indicated that 19 (33.3%) OBI patients and 28 (28%) controls expressed HLA-A2 antigen on their PBMCs. There was no significant difference between the two groups regarding the rate of individuals expressing HLA-A2 antigen. Statistical analyses showed that the intensity of HLA-A2 expression significantly decreased in OBI patients (3.58 ± 0.1) in comparison to healthy controls (4.21 ± 0.25; P 0.001). Conclusions: According to these results it can be concluded that decreased intensity of HLA-A2 on the PBMCs of OBI patients may lead to resistance of HBV in the patients.
Keywords :
Occult , Hepatitis B , Infection , HLA , A2 , DNA ,
