Author/Authors :
Mahmoudi, MR shahid beheshti university of medical sciences - Faculty of Medicine - Department of Parasitology and Mycology, تهران, ايران , Mahmoudi, MR shahid beheshti university of medical sciences - Cellular and Molecular Biology Research Center, تهران, ايران , Mahmoudi, MR guilan university of medical sciences - Fasciolosis Parasitic disease Center Research, رشت, ايران , Ashrafi, K guilan university of medical sciences - Fasciolosis Parasitic disease Center Research, رشت, ايران , Abedinzadeh, H Tehran Province Water Wastewater (TPWW), ايران , Tahvildar-Bideruni, F shahid beheshti university of medical sciences - Faculty of Medicine - Department of Parasitology and Mycology, تهران, ايران , Haghighi, A shahid beheshti university of medical sciences - Faculty of Medicine - Department of Parasitology and Mycology, تهران, ايران , Bandehpour, M shahid beheshti university of medical sciences - Cellular and Molecular Biology Research Center, تهران, ايران , Bandehpour, M shahid beheshti university of medical sciences - Faculty of Medicine - Department of Biotechnology, تهران, ايران , Taghipour Lailabadi, N shahid beheshti university of medical sciences - Faculty of Medicine - Department of Parasitology and Mycology, تهران, ايران , Kazemi, B shahid beheshti university of medical sciences - Faculty of Medicine - Department of Parasitology and Mycology, تهران, ايران , Kazemi, B shahid beheshti university of medical sciences - Cellular and Molecular Biology Research Center, تهران, ايران , Kazemi, B shahid beheshti university of medical sciences - Faculty of Medicine - Department of Biotechnology, تهران, ايران
Abstract :
Background: The protozoan parasites Cryptosporidium spp. and Giardia are known to occur widely in both raw and drinking waters. They are two of the causative agents of waterborne outbreaks of gastroenteritis throughout the world. In the present study, a PCR assay and FA were developed for detection of Cryptosporidium oocysts and Giardia cyst in environmental samples. Methods: We have detected Cryptosporidium spp. oocysts and Giardia cysts in seeded and unseeded environmental water samples by PCR method. Water samples were spiked with oocysts (50, 100,300,500) and filtrated with a 1.2-μm pore size cellulose nitrate and follow by DNA extraction and purification by QIAamp DNA mini kit. Nested-PCR assay amplified an 850 bp fragment of 18s rRNA gene specific for Cryptosporidium and 435 bp fragment of glutamate dehydrogenase (GDH) target gene for Giardia. Also many river water from north of Iran, be checked by these methods. Results: Cryptosporidium and Giardia DNAs were detected in seeded water sample and Giardia was detected in all 5 water samples from river in north of Iran by nested-PCR and FA. Also in one river water sample, Cryptosporidium was detected. Conclusion: This protocol is effective for detection of these waterborne parasites in treated and untreated water samples. This study can also serve as a platform for further investigations and research water source in Iran.
