Title of article :
Chemical Analysis, Antioxidant, Antimicrobial and Enzyme Inhibitory Effects of Daucus Virgatus (Poir.) Maire Essential oil from Algeria
Author/Authors :
Azi, Mouna Laboratory of Chemistry - Engineering - Materials and Nanostructure - University of Ferhas Setif 1, 19000, Algeriaat Abb , Zaidi, Farouk Laboratory of Chemistry - Engineering - Materials and Nanostructure - University of Ferhas Setif 1, 19000, Algeriaat Abb , Sobhi, Widad Laboratory of Applied Biochemistry - Faculty of Life and Nature Sciences - University of Ferhat Abbas Setif 1, 19000, Algeria - Center for Research in Biotechnology - C.R.Bt, P. O. Box: E73/UV N 03 - Ali Mendjeli Nouvelle Ville. Constantine, 25000, Algeria , Flamini, Guido Department of Pharmacy - University of Pisa - Via Bonanno 6, 56126 Pisa, Italy , Bouskout, Mohammed Laboratory of Microbial Biotechnologies - Agrosciences and Environment - Faculty of Sciences Semlalia - Cadi Ayyad University - 40000 Marrakesh, Morocco , Laouer, Hocine Laboratory of Natural and Biological Resources Valorization - Faculty of Life and Nature Sciences - University of Ferhat Abbas Setif 1, 19000, Algeria , Bentouhami, Embarek Laboratory of Chemistry - Engineering - Materials and Nanostructure - University of Ferhas Setif 1, 19000, Algeriaat Abb
Abstract :
Essential oils (EO) represent an important source of bioactive molecules and are widely used for their great efficacy relating to their
different therapeutic properties. The present study aims to contribute to the valorization of Daucus virgatus (Poir.) Maire, an aromatic and
native plant spontaneously growing in the North-Eastern regions of Algeria, by analyzing the chemical composition of its essential oil and
evaluating the antioxidant and antimicrobial activities, together with the assay of the enzyme inhibitory effects against α-glucosidase and
cholinesterase. Daucus virgatus EO was extracted by hydrodistillation and analyzed by gas chromatography-mass spectroscometry analysis
(GC-MS). Twenty-one constituents accounting for 98% of the whole components were identified. β-Pinene (77.9%) and α-pinene (7.6%)
were the most abundant components. The antimicrobial activity against the pathogen microorganisms Listeria innocua, Salmonella
typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Candida albicans was investigated.
The best antimicrobial effect was observed against S. typhimurium and C. albicans, which showed high sensitivity, with inhibition zones
ranging between 27.3 and 25 mm; the minimum inhibitory concentrations were 15.63 and 31.25 μg ml-1, respectively. In the DPPH test, the
essential oil showed a moderate antioxidant effect, with an IC50 value of 39.61 mg ml-1. D. virgatus EO exhibited an interesting inhibitory
effect against α-glucosidase enzyme (IC50 = 0.35 mg ml-1) compared with the positive control Acarbose (IC50 = 0.24 mg ml-1), and a
moderate inhibitory effect against cholinesterases enzymes, with IC50 values of 0.33 and 0.20 mg ml-1 for acetylcholinesterase and
butyrylcholinesterase, respectively. D. virgatus EO might be used as a promising source of natural products with potential antimicrobial,
antihyperglycemic and anti-Alzheimer effects.
Keywords :
Daucus virgatus , Essential oil , Antimicrobial , Anti-cholinesterases , α-Glucosidase inhibition , Antioxidant
Journal title :
Analytical and Bioanalytical Chemistry Research
