Utilizing a Three-phase Hollow Fiber Liquid-phase Micro Extraction Method for Metoprolol Succinate Extraction from Biological Urine Prior to HPLC-UV Analysis

This study presents a three-phase hollow fibers liquid-phase micro extraction (LPME) method for preconcentration of Metoprolol Succinate drugs from tap water and urine samples. The extracted analyte with LPME was detected with high performance liquid chromatography equipped with ultraviolet detector (HPLC-UV). The effect of several parameters on extraction efficiency was investigated and optimized, such as type of organic solvent, receiving phase, pH of receiving phase and extraction phase, extraction time and mixing speed. After extracting the Metoprolol Succinate drug under the optimum conditions, the pre-concentration factor was obtained 192 for tap water and 169 for urine samples with an appropriate detection limit of 3.90 μg l-1 for urine and 1.5 μg l-1 for tap water. The real sample study is provided satisfactory extraction recovery for urine (94%) and tap water (98%) samples.