Author/Authors :
AMMAR, NAGWA M. National Research Centre - Pharmacognosy Department, Egypt , HEFNAWY, MOHAMMED S. Cairo Univeristy - Faculty of Pharmacy - Pharmacognosy Department, Egypt , MOHAMED, DOHA A. National Research Centre - Food Sciences and Nutrition Department, Egypt , KHAMIS, NABIL E. National Research Centre - Tanning Materials and Protein Chemistry Department, Egypt , AFIFI, AHMED H. National Research Centre - Pharmacognosy Department, Egypt , MABRY, TOM J. Texas University - School of Biological Science - Molecular cell and Developmental BiologyDepartment, USA
Abstract :
This study aimed to the discovery of new biologically active natural products with hormone- like activity from Butea frondosa Roxb., Family Leguminosae growing in Egypt. The investigation of estrogen- like activity showed that both the successive non-polar and methanolic extracts showed a significant estrogen-like activity in immature female rats. GLC analysis the non-polar bioactive revealed the presence of eicosane (22.5%) and β-amyrin (20.5%) as the major components in the unsaponifiable matter, while palmitic (24.9%) and linoleic (36.8%) acids were the main saturated and unsaturated fatty acids. The successive bioactive methanolic extract was subjected to chromatographic separation to yield five flavonoids identified as vicenin II, vitexin, chrysoeriol 7-o-β-D-glucuronic acid, 6,8-di-C-rhamnosyl apigenin and luteolin.
