The Study Of Steroid Hormone Effects on the Rate of Growth and Gruit Body Formation in Pleurotus florida (Fr.)Sing

In this study we examined effects of the different concentrations of 17- estradiol and progesterone (0(control), 1.5, 3.02, 6.05 M) on the growth rate and fruit body formation of Pleurotus florida. We cultured Pleurotus florida in Potato Dextrose Agar (PDA) medium for 20 days and measured the diameters of colonies after the 6th and 7th day of inoculation. Then protein content was measured by Lowry Method and protein profile determined by SDS-Page. The results showed that the estroidal (estradiol) treatments increased the rate of growth in comparison with the control. Also the results showed that in 6.05 M progesterone treatment, the colony’s diameters were higher than the other treatments. In 1.5 M estradiol, fruit body formation was stimulated on the 12th day after treatment. In this treatment (1.5 M estradiol) we showed that protein content was higher than the other samples. In the different hormonal treatments we showed vertical growth besides horizontal growth. The Gel electrophoresis of proteins showed that some polypeptide bands with low molecular weight were absent in the different steroid treatment