Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique

Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.