Title of article :
A Blind Smear From LJ Medium Revealing Pseudomonas aeruginosa Talus Osteomyelitis After Seeking for Osteotuberculosis in a 13-Year-Old Teenager: A Case Report
Author/Authors :
Eftekhari ، Nasim Department of Microbiology - Islamic Azad University, Kerman Branch , Rezaeizadeh ، Reza Private Clinic , Shahabinejad ، Shayan Department of Microbiology - School of Medicine - Kerman University of Medical Sciences
Abstract :
The Lowenstein-Jensen is an egg-based microbiology medium that was designed for selective isolation of Mycobacterium spp. The main inhibitory composition in this medium is a synthetic dye called malachite green. Mycobacterium spp. is resistant to the dye by de-colorization and oxidation of it. Some non-tuberculosis organisms may also overcome this inhibitory ingredient by similar enzymatic activity. In the final inspection of the bone TB culture of a teenage patient, the blind smear revealed the Pseudomonas talus osteomyelitis. According to the present and similar experiences, the preparation of 1-2 blind smears from TB culturenegative specimens taken from sterile sites of the body may unmask mycobacteria with the atypical colony and non-tuberculosis microorganisms.
Keywords :
Talus , Lowenstein Jensen (LJ) , Pseudomonas , Osteomyelitis , Malachite green
Journal title :
Case Reports in Clinical Practice
Journal title :
Case Reports in Clinical Practice
