Elevated Secretion of Aldosterone Increases TG/HDLC Ratio and Potentiates The Ox-LDL-Induced Dysfunction of HUVEC

Objective Atherosclerosis (AS) is one of the most common causes of human death and disability. This study is designed to investigate the roles of aldosterone (Aldo) and oxidized lowdensity lipoprotein (OxLDL) in this disease by clinical data and cell model. Materials and Methods In this experimental study, clinical data were collected to investigate the Aldo role for the patients with primary aldosteronism or adrenal tumors. Cell viability assay, fluorescenceactivated cell sorting (FACS) assay, apoptosis assay, cell aging analysis, and matrigel tube formation assay were performed to detect effects on human umbilical vein endothelial cells (HUVECs) treated with Aldo and/or OxLDL. Quantitative polymerase chain reaction (qPCR) and Western blot analysis were performed to figure out critical genes in the process of endothelial cells dysfunction induced by Aldo and/or OxLDL.Results We found that the Aldo level had a positive correlation with the TG/HDLC ratio. Endothelial cell growth, angiogenesis, senescence, and apoptosis were significantly affected, and eNOS/Sirt1, the value of Bcl2/Bax and Angiopoietin1/2 were significantly affected when cells were cotreated by Aldo and OxLDL.ConclusionElevated Aldo with high OxLDL together may accelerate the dysfunction of HUVEC, and the OxLDL, especially for those patients with high Aldo should be well controlled. The assessment of the role of Aldo may provide a theoretical basis for the effective prevention and investigation of a new treatment of AS.