Author/Authors :
Liu ، Bin Department of Instrument Science and Engineering - Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, School of Electronic Information and Electrical Engineering, National Center for Translational Medicine, Collaborative Innovational Center for System Biology - Shanghai Jiao Tong University , Qiao ، Guanglei Department of Oncology - Tongren Hospital - Shanghai Jiao Tong University School of Medicine , Cao ، Wen Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders - Bio-X Institutes - Shanghai Jiao Tong University , Li ، Chenlu School of Laboratory Medicine and Life Science, University Town - Wenzhou Medical University , Pan ، Shaojun School of Biomedical Engineering - Shanghai Jiao Tong University , Wang ، Lirui Department of Instrument Science and Engineering - Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, School of Electronic Information and Electrical Engineering, National Center for Translational Medicine, Collaborative Innovational Center for System Biology - Shanghai Jiao Tong University , Liu ، Yanlei Department of Instrument Science and Engineering - Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, School of Electronic Information and Electrical Engineering, National Center for Translational Medicine, Collaborative Innovational Center for System Biology - Shanghai Jiao Tong University , Ma ، Lijun Department of Oncology - Tongren Hospital, School of Medicine - Shanghai Jiao Tong University , Cui ، Daxiang Department of Instrument Science and Engineering, Department of Oncology - Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, School of Electronic Information and Electrical Engineering, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Tongren Hospital, School of Medicine - Shanghai Jiao Tong University
Abstract :
Objective We aimed to identify the differentially expressed proteins (DEPs) and functional differences between exosomes derived from mesenchymal stem cells (MSCs) derived from umbilical cord (UC) or adipose tissue (AD). Materials and Methods In this experimental study, the UC and AD were isolated from healthy volunteers. Then, exosomes from UCMSCs and ADMSCs were isolated and characterized. Next, the protein compositions of the exosomes were examined via liquid chromatography tandem mass spectrometry (LCMS/MS), followed by evaluation of the DEPs between UCMSC and ADMSC–derived exosomes. Finally, functional enrichment analysis was performed. Results One hundred and ninetyeight key DEPs were identified, among which, albumin (ALB), alphaIIspectrin (SPTAN1), and Rasrelated C3 botulinum toxin substrate 2 (RAC2) were the three hub proteins present at the highest levels in the proteinprotein interaction network that was generated based on the shared DEPs. The DEPs were mainly enriched in gene ontology (GO) items associated with immunity, complement activation, and protein activation cascade regulation corresponding to 24 pathways, of which complement and coagulation cascades as well as platelet activation pathways were the most significant. ConclusionThe different functions of AD and UCMSC exosomes in clinical applications may be related to the differences in their immunomodulatory activities.
Keywords :
Complement and Coagulation Cascades , Exosomes , Mesenchymal Stem Cells , Proteomics Analysis ,
