High throughput analysis on selected polyphenol production and Principal Component Analysis (PCA) in Phyllanthus watsonii grown under different environmental conditions

Polyphenol production in Phyllanthus watsonii (species of Dukung anak) planted in different environmental conditions was investigated. The plants were grown in two different environments: A – in the net house under semi-controlled environmental condition, and B – in an open environment. Environmental parameters such as light intensity (kLux), soil moisture content (%), relative humidity (% RH) and surrounding temperature (°C) were taken on daily basis for comparison. Water extract of the samples showed that P. watsonii planted in the open environment yielded higher amounts of polyphenols compared to that planted in semi-controlled environment. However, there was significant inconsistency (p 0.05) in the polyphenol yield among the triplicates of P. watsonii planted in the open environment compared to that of the semicontrolled environment. Statistical results from principal component analysis (PCA) and quantifications of four known phenolic acids (geraniin, corilagin, rutin and quercetin glucoside) from LCMS/MS verified these findings. The findings revealed that polyphenol production in P. watsonii can be significantly influenced under different environmental conditions