Author/Authors :
Khatami, Mehrdad Noncommunicable Diseases Research Center - Bam University of Medical Sciences - Bam, Iran - Cell Therapy and Regenerative Medicine Comprehensive Center - Kerman University of Medical Sciences - Kerman, Iran , Khatami, Sanaz Department of Medical Biotechnology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran, Iran , Mosazade, Farideh Noncommunicable Diseases Research Center - Bam University of Medical Sciences - Bam, Iran , Raisi, Mahammadali Clinical Research Center - Pastor Educational Hospital - Bam University of Medical Sciences - Bam, Iran , Haghighat, Mojtaba Behbahan Faculty of Medical Sciences - Behbahan, Iran , Sabaghan, Mohamad Behbahan Faculty of Medical Sciences - Behbahan, Iran , Yaghoubi, Sajad Department of Clinical Microbiology - Iranshahr University of Medical Sciences - Iranshahr, Iran , Sarani, Mina Zabol Medicinal Plants Research Center - Zabol University of Medical Sciences - Zabol, Iran , Bamorovat, Mehdi Leishmaniasis Research Center - Kerman University of Medical Sciences - Kerman, Iran , Malekian, Leila Clinical Research Center - Pastor Educational Hospital - Bam University of Medical Sciences - Bam, Iran , Naroi, Afsoon Student Research Committee - School of Public Health - Bam University of Medical Sciences - Bam, Iran , Varma, Rajender S. Regional Centre of Advanced Technologies and Materials Faculty of Science - Palacky University in Olomouc - Olomouc, Czech Republic
Abstract :
Background: NPs (NPs) with unique chemical and physical properties can be used for therapeutic purposes because of their strong antimicrobial activates. NPs have been used as an antimicrobial agents to inhibit microbial growth.
Objectives: In view of the strong antimicrobial activity of NPs, the biogenic synthesis and leishmanicidal activity of rod-shaped zinc oxide (R-ZnO) NPs was explored using Lilium ledebourii tuber extract.
Materials and Methods: The ensuing NPs are characterized by UV-visible spectroscopy, X-ray diffraction and transmission electron microscopy and their leishmanicidal activity evaluated against the Leishmania major (L. major) by MTT assay.
Results: The R-ZnO NPs displayed excellent leishmanicidal activity against the L. major as they significantly inhibited the amastigotes. The IC50 values of R-ZnO NPs being ~ 0.001 mg.mL-1. R-ZnO NPs can inhibit L. major growth in a dose-dependent manner under in vitro conditions.
Conclusion: A simple, low-cost feasible and eco-friendly procedure was developed for biosynthesis of R-ZnO NPs using natural bioresource that can inhibit human parasite cells growth in a dose-dependent manner under in vitro conditions.
Keywords :
Biosynthesis , Leishmanicidal , NPs , Rod-shaped , Zinc Oxide
