Author/Authors :
Salhi, Imed Livestock and wildlife laboratory - Arid lands Institute - Gabès University - Medenine, Tunisia , Bessalah, Salma Livestock and wildlife laboratory - Arid lands Institute - Gabès University - Medenine, Tunisia , Snoun, Dalila Livestock and wildlife laboratory - Arid lands Institute - Gabès University - Medenine, Tunisia , Khorchani, Touhami Livestock and wildlife laboratory - Arid lands Institute - Gabès University - Medenine, Tunisia , Hammadi, Mohamed Livestock and wildlife laboratory - Arid lands Institute - Gabès University - Medenine, Tunisia
Abstract :
Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.
Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies, to target pathogenic E. coli surface antigens.
Material and methods: We immunized a camel with a killed strain we had previously isolated from a diarrheic camel calf and identified as expressing the F17 fimbriae antigen.
Results: The immunized animal developed an anti-E.coli immune response including heavy-chain antibodies. Lymphocytes from this animal were purified and RNA isolated to create a VHH library by phage display with a size of about 109 individual transformants. Panning on live E. coli cells resulted in the isolation of VHH fragments specific to the cell surface antigens.
Conclusion: The identification of these antigens can lead to the development of new diagnostic and therapeutic tools against diarrhea.
Keywords :
Escherichia coli , diarrhea , nanobodies , phage display
