Title of article :
Exposing Mouse Oocytes to MitoQ During In Vitro Maturation Improves Maturation and Developmental Competence
Author/Authors :
Hosseinzadeh Shirzeyli, Maryam Department of Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Amidi, Fardin Department of Anatomical Sciences - School of Medicine - Tehran University of Medical Sciences - Tehran, Iran , Shamsara, Mehdi Department of Animal and Marine Biotechnology - National Institute of Genetic Engineering and Biotechnology (NIGEB) - Tehran, Iran , Nazarian, Hamid Department of Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Eini, Fatemeh Fertility and Infertility Research Center - Hormozgan University of Medical Sciences - Bandar Abbas, Iran , Hosseinzadeh Shirzeyli, Farhad Department of Animal Science - Faculty of Agriculture and Natural Resources - Science and Research branch - Islamic Azad University - Tehran, Iran , Majidi Zolbin, Masoumeh Department of Anatomical Sciences - School of Medicine - Tehran University of Medical Sciences - Tehran, Iran , Ghaffari Novin, Marefat Cellular and Molecular Biology Research Center - School of Medicine - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Daliri Joupari, Morteza Department of Animal and Marine Biotechnology - National Institute of Genetic Engineering and Biotechnology (NIGEB) - Tehran, Iran
Abstract :
Background: Mitochondrion is the main indicator of oocyte quality and one of the components of oocyte, which is sensitive
to oxidative damage during the maturation process. Mitoquinone mesylate (MitoQ) is a strong antioxidant targeting
mitochondria as well as anti-apoptotic agent. However, the effect of MitoQ on the quality of oocytes during in vitro maturation
(IVM) is still unknown.
Objective: This study investigated the possible effects of MitoQ on maturation and developmental competency in mice
oocytes.
Materials and Methods: The oocytes were collected at germinal vesicle stage from 6-8-week old female NMRI mice and
then cultured in TCM-199 medium supplemented with 0, 0.01, 0.02 and 0.04 μM MitoQ. The sham group was treated with
DMSO (0.01% v.v). Then intracellular Glutathione (GSH), reactive oxygen species (ROS) levels, mitochondria membrane
potential (ΔΨm), as well as in vitro fertilization (IVF) rate in the 18-20 h matured oocytes and metaphase II (MII) oocytes
(in vivo-control), were assessed.
Results: The results showed that between three dose of MitoQ, the 0.02 μM significantly increased nuclear maturation rate,
GSH level, fertilization rate and blastulation (92.6, 231.7, 90.19 and 81.66%, respectively) than the in vitro-control (71.14,
152, 78.84 and 73.50%, respectively) and more comparable to that of the in vivo matured oocytes (100, 243.5, 92.10 and
83%, respectively). Also, the mitochondria membrane potential in the 0.02 μM MitoQ was significantly higher compared
with those in the other groups (4.4). However, the intracellular ROS level in 0.02 μM MitoQ was significantly decreased
(38.72%) compared to in vitro-control (82.2%) and was similar to the in vivo-control (33.5%).
Conclusion: The results indicated that supplementation of IVM medium with MitoQ (specially 0.02 μM) enhance maturation
and fertilization rate. In conclusion, MitoQ might be considered as a novel component that could be added to IVM media.
Keywords :
Antioxidant , Assisted reproduction , Fertilization , MitoQ , Oocyte in vitro maturation
Journal title :
Iranian Journal of Biotechnology (IJB)
