Title of article :
Acrylamide exposure aggravates the development of ulcerative colitis in mice through activation of NF-κB, inflammatory cytokines, iNOS, and oxidative stress
Author/Authors :
Amirshahrokhi ، Keyvan Department of Pharmacology - School of Pharmacy - Ardabil University of Medical Sciences
From page :
312
To page :
321
Abstract :
Objective(s): Acrylamide is a toxic compound that forms during food processing at high temperatures. Acrylamide has been shown to induce toxicity in various organs in the body. This study aimed to investigate the effect of acrylamide exposure on the susceptibility of the colon to ulcerative colitis in a mouse model. Materials and Methods: Mice were pretreated with acrylamide (oral, 20 and 30 mg/kg/day) for 21 consecutive days, and colitis was induced by intrarectal administration of acetic acid. Results: The results revealed that acrylamidepretreatment significantly increased disease activity index (DAI), macroscopic damage, histological changes of the colonic mucosa and oxidative stress markers carbonyl protein, malondialdehyde (MDA), and nitric oxide (NO), whereas it decreased the levels of antioxidants glutathione (GSH), superoxide dismutase (SOD) and catalase. Moreover, induction of colitis in acrylamidepretreated mice caused a higher increase in colonic levels of myeloperoxidase (MPO), matrix metalloproteinase (MMP)9, monocyte chemoattractant protein (MCP)-1, cytochromec, caspase3, proinflammatory cytokine tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and interferon (IFN)-γ, whereas it reduced the level of IL-10. The mRNA expression of nuclear factor kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) were further increased in colon tissue of mice exposed to acrylamide.Conclusion: These findings suggest that acrylamide can accelerate the development of acetic acidinduced colitis. In conclusion, chronic acrylamide exposure may aggravate the severity of ulcerative colitis and increase colonic mucosal damage through oxidative stress and inflammatory responses.
Keywords :
Acrylamide , Apoptosis , Cytokines , Inflammation , Oxidative stress , Ulcerative colitis
Journal title :
Iranian Journal of Basic Medical Sciences
Journal title :
Iranian Journal of Basic Medical Sciences
Record number :
2578942
Link To Document :
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