Title of article :
ELEVATED TEMPERATURE REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF POLICYCLIC AROMATIC HYDROCARBONS ON ODS-SILICA PHASE
Author/Authors :
SALEH, NOORASHIKIN MD Universiti Malaysia Terengganu - Faculty of Science and Technology - Department of Chemical Sciences, Malaysia , SANAGI, M. MARSIN Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia , IBRAHIM, WAN AINI WAN Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia , NAIM, AHMEDY ABU Universiti Teknologi Malaysia - Faculty of Science - Department of Chemistry, Malaysia
Abstract :
Reversed-phase high performance liquid chromatography (RP-HPLC) was used at various temperatures (room temperature to 70°C) to separate Polycyclic aromatic hydrocarbons (PAHs) on silica ODS using proportions of organic modifier in the mobile phase. The selectivity and retention patterns of four PAHs (naphthalene, fluorene phenanthrene and fluoranthene) were investigated. Successful separations with high column efficiencies were achieved at high column temperatures. Selected separation conditions for all PAHs compounds at elevated temperature were: mobile phase: acetonitrile-water: 40-60 v/v, column: Hypersil ODS, 5|a (4.6mm I.D. x 100mm), flow rate: 2.5 mL/min, UV wavelength at 254 nm.
Keywords :
Polycyclic Aromatic Hydrocarbons (PAHs) , high temperature HPLC , silica column
Journal title :
Journal of Sustainability Science and Management
Journal title :
Journal of Sustainability Science and Management
