Author/Authors :
Salehi, A. imam hossein university - Faculty of Basic Sciences - Department of Cell Molecular Biology, تهران, ايران , Saadati, M. imam hossein university - Faculty of Basic Sciences - Department of Cell Molecular Biology, تهران, ايران , Raoofian, R. tehran university of medical sciences tums - Faculty of Medicine - Department of Medical Genetics, تهران, ايران , Bahador, A. tehran university of medical sciences tums - Faculty of Medicine - Department of Microbiology, تهران, ايران , Doroudian, M. islamic azad university - Young Researchers Club, ايران , Ahsanim, Y. imam hossein university - Faculty of Basic Sciences - Department of Cell Molecular Biology, تهران, ايران , Hosseini, M. baqiyatallah university of medical sciences - Research Center of Applied Microbiology, ايران , Heidari, M. tehran university of medical sciences tums - Faculty of Medicine - Department of Medical Genetics, تهران, ايران
Abstract :
Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene deletion are advised candidates of vaccine against shigella. The ipaD gene is responsible for Shigella invasion which act as a chaperone for some other invasion proteins such as IpaB and IpaC. We used λ-red recombination system to delete the ipaD gene in shigella that end to production of a live attenuated form of Shigella dysenteriae. This strain has a high prevalence in Iran that make it suitable target for vaccine production. Our results showed that live attenuated ΔipaD Shigella was produced via λ-red recombination system. We suggest that application of λ-red recombination could be a simple and useful technique for production of live attenuated ΔipaD Shigella as a candidate of vaccine against shigellosis
Keywords :
Gene deletion ، Shigella dysenteriae , λ-red Recombination , ipaD , Attenuated live vaccine
