Effect of Some Technical Processes on Deep- Freezing, Enzyme Leakage and Fertility of Buffalo Spermatozoa

THE PRESENT study was performed to clarify the effect of 2 diluents (Tris and Milk), 2 cooling rates (35°C to 5°C for 1 and 2 hr), 2 equilibration periods (1 and 2 hr) and their interactions on the freezability, alkaline phosphatase (AKP), Glutamic oxaloacetic transaminase (GOT) leakages and fertility of frozen-thawed semen in 24 ejaculates from 4 buffalo bulls.The present study revealed that the pre- and post-freezing motility, acrosomal integrity, AKP, GOT leakages and fertility rate were significantly (P 0.01) influenced by different cooling rates equilibration periods, diluents and their interactions.The mean pre-freeze and post-thawing motility following 2 hr of cooling was significantly higher (P 0.01) than that following 1 hr Whereas, the mean pre-freeze and post-thawing acrosomal defects following 2 hr of cooling was significantly less (P 0.01) than that following 1 hr. The leakage of GOT was significantly lower (P 0.01), leakage of AKP was significantly higher (P 0.01) and the conception rates were higher for semen frozen after 2 hr (64.8%) than from 1 hr (38.4%)