Author/Authors :
Nazemalhosseini-Mojarad, Ehsan shahid beheshti university of medical sciences - Research Institute for Gastroenterology and Liver Disease, تهران, ايران , Azimirad, Masoumeh shahid beheshti university of medical sciences - Research Institute for Gastroenterology and Liver Disease, تهران, ايران , Razaghi, Maryam shahid beheshti university of medical sciences - Research Institute for Gastroenterology and Liver Disease, تهران, ايران , Torabi, Parisa shahid beheshti university of medical sciences - Research Institute for Gastroenterology and Liver Disease, تهران, ايران , Moosavi, Ali shahid beheshti university of medical sciences - Research Institute for Gastroenterology and Liver Disease, تهران, ايران , Alebouyeh, Masoud shahid beheshti university of medical sciences - Research Institute for Gastroenterology and Liver Disease, تهران, ايران , Aslani, Mohammad Mehdi Pasteur Iinstitute of Iran - Deprtment of Microbiology, تهران, ايران , Zali, Mohammad Reza
Abstract :
Aim: In this study, the prevalence of C. difficile, from patients with gastrointestinal complaints and its association with other enteropathogen microbes were investigated. Background: Clostridium difficile is an important pathogen associated with outbreaks of pseudomembranous colitis and other intestinal disorders, such as diarrhea. Patients and methods: Enterotoxin and cytotoxin (toxin A and toxin B) of C. difficile on the patient’s stool samples were detected by a double sandwich enzyme-linked Immunosorbant assay technique using a commercial kit (Premier toxins A B; Generic Assays, Inc., Germany). The microbial isolation and examination was done, according to the standard identification methods. Results: Out of 356 individuals (57.6 % male and 42.4 % female) the results of C. difficile were positive for 19 patients (5.3 %) and negative for 337 patients (94.6 %) according to the results of C. difficile antigen kit. There was no association between the existence of C. difficile toxin and microbial population or antibiotic usage. Conclusion: This prevalence study clearly supports the hypothesis of a probable role of C.difficile in developing gastrointestinal complaints in patients with diarrhea. More studies are needed to evaluate the role of C. difficile in these diseases.
