Title of article :
Cross reactivity between ES and somatic antigens of Fasciola spp in enzyme linked immunosorbent assay.
Author/Authors :
Moazeni, M. shiraz university - School of Veterinary Medicine - Department of Pathobiology, شيراز, ايران , Gaur, S. N. S. shiraz university - School of Veterinary Medicine - Department of Pathobiology, شيراز, ايران , Shahangian, A. shiraz university - School of Veterinary Medicine - Department of Pathobiology, شيراز, ايران
Abstract :
Excretory-secretory (ES) and somatic antigens of Fasciola hepatica and Fasciola gigantica were prepared from freshly collected flukes. Laboratory bred rabbits were immunizedwith antigens for preparation of antisera. ES antigens of both species showedstrong positive reaction with antisera raised againstES and somatic antigens of parasite. Somatic antigens of both species also showed strong positive reactionwith antisera raised against somatic and ES antigens of parasite. In homologouscombinationof antigensand antisera higher enzyme linked immunosorbent assay (ELISA) values was observed in comparison with heterologous combination, so it was concluded that ES and somatic antigens of Fasciola spp have strong cross reaction with each other but the antigenic materials of ES and somatic products of parasite are not completely the same.
Keywords :
Fasciola , ELISA , ES antigen , Somaticantigen
Journal title :
Iranian Journal of Veterinary Research (IJVR)
Journal title :
Iranian Journal of Veterinary Research (IJVR)
