Author/Authors :
Ghafari, Sh. shahid chamran university of ahvaz - Faculty of Sciences, اهواز, ايران , Seyfiabad Shapouri, M. R. shahid chamran university of ahvaz - Faculty of Veterinary Medicine - Department of Pathobiology, اهواز, ايران , Moatamedi, H. shahid chamran university of ahvaz - Faculty of Sciences - Department of Biology,, اهواز, ايران , Roayaei, M. shahid chamran university of ahvaz - Faculty of Sciences - Department of Biology, اهواز, ايران , Goudarzi, H Razi Vaccine and Serum Research Institute - Department of Avian Medicine, ايران
Abstract :
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was cloned in a eukaryotic expression vector, pSec Tag2A. The gene was placed down stream of Ig (Kappa) chain leader sequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. The construct pSec Tag 2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 was assessed by dot blotting and anti gen capture ELISA. The antibody used in the immunological assays was aneutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated the construct was functional with respect to expression and secretion of a native VP2
Keywords :
Infectious bursal disease , IBDV , VP2 , Eukaryotic expression , COS , 7 cell line
