Effects of Diazepam and Xylazine on Changes of Blood Oxygen and Glucose Levels in Mice

The present study aimed to explore the changes of body temperature, heart rate, blood partial pressure of oxygen (PO2) and glucose levels upon diazepam and/or xylazine administration in mice. Sixty male albino mice (33.89 ± 0.41 g body weight; BW) were distributed randomly into five groups given intraperitoneal (IP) injection: control group given physiological saline; diazepam group (D) given13.3 mg/kg BW; xylazine group (X) given26.6 mg/kg BW); DX group given both13.3 mg/kg BW diazepam and 26.6 mg/kg BW xylazine; DXVas group given DX dose for vasectomy surgical operation. The values of body temperature, PO2, heart rate and blood glucose were recorded of groups at 0, 20 min, 40 min, 1h, 2h, 3h, 4h, 8h of diazepam and/or xylazine injection. The results indicated a significant (P < 0.05) transient negative side effect of diazepam and/or xylazine dosages on body temperature, PO2, heart rate and blood glucose, which were the highest at 1-2 h post-drug administration. The transient negative side effects decreased gradually thereafter at 2h, 3h, 4h and 8hof drugs’ injection. The transient negative side effects were more significantly pronounced in xylazine, DX and DXvas groups compared to diazepam and control groups and extended to 8h of injection. In conclusion, the given dosage of diazepam and/or xylazine resulted in transient negative side effects in body temperature, PO2, heart rate and blood glucose, which returned approximately close to normal levels at 8h of injection. The DX anesthesia dose is sufficient and safe for performing minor and major surgeries in mice.