Anti-inflammatory, Antinociceptive and Nephroprotective activities of Tilia cordata and Isolation of Bioactive Compounds

Many studies aim to manage many diseases by discovering new drugs from medicinal plants. This study was designed to evaluate anti-inflammatory, antinociceptive and nephroprotective activities of 70% methanol extract of Tilia cordata aerial parts and to isolate and identify its phytoconstituents. Antiinflammatory activity was evaluated by using the carrageenan-induced rat paw edema method, antinociceptive activity was evaluated by using the writhing test in mice, nephroprotective activity against the toxic effect of cadmium chloride (CdCl2) on the renal function was determined by measuring creatinine and uric acid levels. Moreover, the study showed the isolation and identification of the bioactive compounds using chromatographic and spectrophotometric methods .Six phenolic compounds were isolated which were identified as; kaempferol, quercetin, vitexin, kaempferol 3-O-α-rhamnoside, quercetin 3-O-β-galactoside and kaempferol 3-Orutinoside. The extract of Tilia cordata showed a powerful anti-inflammatory, antinociceptive and nephroprotective activities. This research aims to enter the field of drug discovery by using the methanol extract of Tilia cordata aerial parts in the treatment of inflammation, pain and nephrotoxicity.