مرکز منطقه ای اطلاع رساني علوم و فناوري - Modulatory Effects of Casimiroa Edulis on Aluminium Nanoparticles - Associated Neurotoxicity in A Rat Model of Induced Alzheimer s Disease

Author/Authors :

aly, h.f. national research centre - department of therapeutic chemistry, Giza, Egypt , elrigal, n. s. national research centre - department of therapeutic chemistry, Giza, Egypt , ali, s. a. national research centre - department of therapeutic chemistry, Giza, Egypt , rizk, m. z. national research centre - department of therapeutic chemistry, Giza, Egypt , ebrahim, n. a. national research centre - department of pharmacognosy, Giza, Egypt

Abstract :

This study evaluated the biological effectiveness of aluminum oxide nanoparticles (Al_2O_3NPs), on brain rats by analyzing brain function neurotransmitters, oxidative stress and antioxidant biomarkers. Rats were administered Al2O3 nanoparticles (NPs)of 20 nm diameter at dose 50 mg/kg body weight and examined for induction of Alzheimer s disease (AD) in rats model through determination of acetylcholine esterase (ACHE), noradrenaline (NA), adrenaline (A), serotonin , dopamine , apoptotic markers; caspase -3, oxidative stress, lipid peroxide (MDA), nitric oxide (NO) antioxidant markers , glutathione reduced (GSH), total antioxidant capacity (TAC) and calmoduline (CaM) . Exposure to Al2O3 NPs induced AD in rats showed significant reduction in NA, A with percentages decrease 42.18 and 34.09%, respectively , while significant increase in ACHE activity (64.83%), as compared to normal control rats. However AD induced rats showed significant reduction in serotonin and dopamine with percentages decrease 38.17and 54.02%, respectively as compared to normal control rats. However, Al NPS intoxicated rats showed significant increase in caspase -3 with percentages increase 99.10%. However, Al NPS induced rats showed significant decrease in TAC and GSH levels with percentages 59.70 and 61.00%, respectively. While, significant increase in CaM(+206.09%), lipid peroxide (108.74%) and NO (74.43%) levels as compared to normal control rats was detected. Histopathological investigation of NPs induced rats’ demonstrated marked amelioration in all parameters under investigation upon treated intoxicated rats with Casimiroa edulis total extract as compared to dopenzil standard drug. These results are conformed by histopathological examination which revealed improvement in brain neuron architectures with various degrees. It could be concluded that Casimiroa edulis total extract may be useful in ameliorating Al NPs induced AD in rats model which may be used as an intelligent candidate nutraceutical.