Seed Treatment With Chitosan and Ethanol-Extracted Propolis for Suppression Bean Root Rot Disease Under Greenhouse Conditions

Root rot disease caused by Fusarium solani, Sclerotium rolfsii and Rhizoctonia solani is a serious and persistent disease problem of bean plants during growing season. The effects of ethanol-extracted propolis (EEP) and chitosan as seed treatments on bean root rot disease under greenhouse conditions was evaluated. Complete inhibition of linear growth was obtained with chitosan at 8.0 g / L concentration. The heights increase of inhibition zone area was obtained with EEP at 15.0 %. Greenhouse experiments revealed that all tested concentrations of chitosan and/or EEP significantly reduced the bean root rot disease caused by R. solani and S. rolfsii. The highest reduction in root rot disease incidence was obtained with chitosan at 8.0 g /L and EEP at 15 % which showed highly significantly reduction in both root rot disease incidence and disease severity. Other treatments showed moderate effect.