Author/Authors :
Dasari, Sreenivasulu Department of Biochemistry - Sri Venkateswara University, Tirupati, India , Swamy Ganjayi, Muni Department of Biochemistry - Sri Venkateswara University, Tirupati, India , Gonuguntla, Sailaja Department of Biochemistry - Sri Venkateswara University, Tirupati, India , Rao Kothapalli, Srinivasa Department of Veterinary Parasitology - College of Veterinary Science - Sri Venkateswara Veterinary University, Tirupati, India , Yellanur Konda, Prabhakar Biochemistry Division - National Institute of Nutrition, Hyderabad, India , Basha, Sreenivasulu Department of Microbiology - Sri Venkateswara University, Tirupati, India , Kutagolla, Peera Department of Zoology - DBT BIF Center - Sri Venkateswara University, Tirupati, India , Meriga, Balaji Department of Biochemistry - Sri Venkateswara University, Tirupati, India
Abstract :
Disturbance of the biomarkers in hepato and nephrotoxicity that induced by acrylamide (ACR) were studied in rat. ACR (50 mg) was administered through drinking water (300 ml) on alternative days up to 27 days, and liver and kidney tissues were collected on 27th day post ingestion for experimentation. The results revealed that ACR causes significant increase in non-enzyme markers such as lipid peroxidation (p˂0.05) and nitric oxide (NO) (p˂0.05), but decrease in glutathione (GSH) (p˂0.05), and similarly enzymemarkers such as glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities were significantly decreased (p˂0.05) at 27th day post ingestion. The histology study revealed that ACR induced degenerative changes in the liver and kidney such as enlarged sinusoid, fragmentation of hepatocyte nuclei, inflammation of Kupffer cells and spreading hepatic contents towards central vein, and tubular thyroidisation, inflammation in distal tubules, distorted glomerulus and proximal tubules fragmentation, respectively. In conclusion, ACR that shows significant effect on liver and kidney biomarkers and leads to liver and kidney tissue degeneration wistar rats.
