Author/Authors :
Atef Kandil, Aya Animal Health Research Institute, Mansoura, Egypt , Elhadidy, Mohamed Department of Bacteriology Mycology and Immunology - Faculty of Veterinary Medicine - Mansoura University, Egypt , El-Gamal, Ahmed Animal Health Research Institute, Mansoura, Egypt , Al-Ashmawy, Maha Abdou Department of Food Hygiene and Control - Faculty of Veterinary Medicine - Mansoura University, Egypt
Abstract :
The present study was performed to determine the prevalence of S. aureus and E. coli in milk and some dairy products sold in Mansoura city, Egypt. A total two hundred samples including kariesh cheese (50 samples), market raw milk, market sterile milk, small scale yoghurt, large scale yoghurt, small scale ice cream, and large scale ice cream samples (25 samples from each product) were collected for microbiological examination. Recovered isolates were identified using an array of biochemical and serological tests for E. coli. Polymerase chain reaction (PCR) was applied to detect the nuc gene as a species-specific marker gene for S.aureus. Our result revealed that E. coli was observed in 86%, 60%, 88%,80%, 92% and 28% of kariesh cheese, market raw milk, small scale yoghurt, large scale yoghurt, small scale ice cream and large scale ice cream samples, respectively.None of the sterile milk samples was recorded for E. coli contamination. The prevalence of S. aureus was 92%, 80%, 36%, 100% and 40%, respectively. Likewise, none of the yoghurt large scale & sterile milk samples was recorded for S. aureus contamination. These findings highlighted the importance of periodical application of strict hygienic measures in dairy plants and markets for monitoring the public health hazards.
Keywords :
E.coli , S.aureus , Milk , Dairy products
