Author/Authors :
Awad, M.H. Sudan University for Sciences and Technology, Sudan , Hassan, E.A. Sudan University for Sciences and Technology, Sudan , Osmon, M.E. Sudan University for Sciences and Technology, Sudan , Alassaf, Saphwan Glyndwr University, UK , Alassaf, Saphwan University of Wolverhampton - School of Applied Sciences, UK , Phillips, Glyn O. University of Wolverhampton - School of Applied Sciences, UK , Phillips, Glyn O. Glyndwr University, UK
Abstract :
The determination of weight average molecular weight (Mw) and radius of gyration (Rg) ofHabeil gum was performed on a gel permeation chromatography-multi angle laser lightscattering (GPC-MALLS) system. The results showed three main components of Combretumglutinosum gum designated arabinogalactan protein (AGP), arabinogalactan (AG) andglycoprotein (GP). The Mw of the freeze dried soluble part, freeze dried deacetylated gum, andfreeze dried calcium-free gum were 2.06×10^6, 8.03×10^5, and 1.40×10^6 g /mol, respectively. The Mw of hydrophilic and hydrophobic components collected from soluble part gum are 3.16×10^6 and 6.08×10^5 g /mol, respectively, while the Mw of hydrophilic and hydrophobic components collected from deacetylated gum are 1.39×10^6 and 8.36×10^5 g /mol, respectively. Emulsification studies of the gum fractions showed lowest degree of span value than the starting material indicating good uniformity and increasing in adsorption of protein on oil droplet surface. The results of emulsion studies indicated that a blend of 50% w/w deacetylated Combretum glutinosum gum and 50% w/w Acacia Senegal produced better emulsification properties than the pure mother gum.
