Author/Authors :
Ardalan, Mohammad Reza tabriz university of medical sciences - Department of Nephrology, ايران , Estakhri, Rasoul tabriz university of medical sciences - Department of Pathology, ايران , Hajipour, Babak urmia university of medical sciences, ايران , Ansarin, Khalil tabriz university of medical sciences - Tuberculosis and Lung Disease Research Center, ايران , Ahmadi Asl, Naser tabriz university of medical sciences - Department of Physiology, ايران , Nasirizade, Mohammad Reza islamic azad university - Faculty of Veterinary Medicine - Department of Physiology, ايران , Nour Azar, Alireza islamic azad university - Faculty of Veterinary Medicine - Department of Physiology, ايران , Ghorbanihaghjou, Amir tabriz university of medical sciences - Department of Biochemistry, ايران , Vatankhah, Amir Mansour tabriz university of medical sciences - Drug Applied Research Center, ايران , Esmaili, Heydar Ali tabriz university of medical sciences - Department of Pathology, ايران
Abstract :
Objective: To study the effect of erythropoietin (EPO) treatment on renal and lung injury following renal ischemia/reperfusion (I/R). Materials and Methods: Thirty male Wistar rats were assigned to three groups of 10 rats each. The first group was sham-operated, the second was subjected to renal I/R (30 min of ischemia followed by 24 h of reperfusion). The third group was subjected to renal I/R and treated with EPO in two doses: the first dose 1 h prior to ischemia (1,000 U/kg) and the second dose 6 h after ischemia (1,000 U/kg). Results: The renal and lung tissue injury index, tissue serum blood urea nitrogen and creatinine (Cr) were higher in the renal I/R group compared to the renal I/R + EPO group; the difference was statistically significant (p 0.05). Kidney and lung tissue glutathione peroxidase and superoxide dismutase levels were higher in the renal I/R + EPO group than the renal I/R group; the difference was also statistically significant (p 0.05). Conclusion: The data showed that EPO pretreatment could be effective in reducing renal and lung injury following renal I/R and could improve the cellular antioxidant defense system. Hence EPO pretreatment may be effective for attenuating renal and lung injury after renal I/R-induced injury during surgical procedures, hypotension, renal transplantation and other conditions inducing renal I/R.
Keywords :
Erythropoietin , Ischemia , reperfusion , Kidney , Lung
