Comparative Pharmacodynamic Effect between Folate Isolated from Spinacia Oleracea Leaves and Synthetic Folic acid on Pregnant Mice

The objective was designed to study a comparative effect between natural folate and synthetic folic acid on pregnant mice. Folate was extracted from Spinacia oleracea leaves; a pilot study was conducted by using different doses (0.123, 0.861, 1.72, 2.5 and 3.44) mg/kg. Sixty mice were divided into four collections, first group (G1) was control group, second group (G2) was treated with folate (2.33) mg/kg of B.W, third group (G3) was treated with folic acid (0.861) mg/kg B.W, fourth group (G4) were treated with extract of Spinacia Oleracea (100) mg/kg B.W. The RBC, Hb, ovaries and uterus weight ratio, folic acid concentration and pregnancy ratio in G2 revealed a significant rise in comparison with G3, G4 and G1. LH, FSH, progesterone and estrogen levels increase statistically in G2 group when compared with G1 and G3. Uterine tissue in G2 showed an increase in the number of endomaterial glands. G4 appeared increase of endometrium thickness, while G3 showed rounded basal nuclei of the endometrial glands. Ovaries tissues in G2 and G4 showed the cortex contained numerous follicles and presence the corpus luteum. G3 revealed maturation of follicles more than G1.The study was concluded the naturally extracted folate had the ability to maintain the pregnancy ratio, RBC, Hb, regulation of reproductive hormones and level concentration of folic acid by metabolized in the small intestine more than synthetic folic acid.