Title of article :
On-Column Approach in the HPLC-UV Analysis of Non-chromophoric Compounds Using Azelaic Acid as a Model
Author/Authors :
Alzweiri, Muhammed University of Jordan - Faculty of Pharmacy - Department of Pharmaceutical Sciences, Jordan , Al-Hiari, Yusuf M. University of Jordan - Faculty of Pharmacy - Department of Pharmaceutical Sciences, Jordan , Aburjai, Talal University of Jordan - Faculty of Pharmacy - Department of Pharmaceutical Sciences, Jordan , Abdel-Aldaem, Osama University of Jordan - Faculty of Pharmacy - Department of Pharmaceutical Sciences, Jordan
Abstract :
The manuscript introduces a detection solution of compounds lacking chromophoric properties (e.g. azelaic acid)by implementing a HPLC-UV analysis with on-column derivatisation of the analyte. Azelaic acid was used totest the feasibility of the method at λmax = 265 nm. Its chromatographic analysis shows linear (R = 0.999),precise (RSD 2.0%) and accurate (97.0 - 103.5 %) behavior. Furthermore, the method was found selective forazelaic acid in a prepared cream which contains other ingredients such as triethanolamine, vaseline and stearicacid. The limit of detection (LOD) and limit of quantification (LOQ) of azelaic acid were 9 and 30 μg/ml,respectively
Keywords :
HPLC , UV , Azelaic acid , On , column derivatisation
Journal title :
Jordan Journal of Pharmaceutical Sciences
Journal title :
Jordan Journal of Pharmaceutical Sciences
