Evaluation of the Starch Quantification Methods of Musa paradisiaca, Manihot esculenta, and Dioscorea tr$fida Using Factorial Experiments

Background. Starch and its products are used in a variety of ways for both the food and nonfood industries. A factorial experiment is carried out with two factors to explain the behavior of the percentage of starch, where the factors correspond to the extraction method and to the raw material. Method. Tree methods were used in triplicate: the frst followed the ofcial technique of the Association of Ofcial Analytical Chemists (AOAC), to perform acid hydrolysis and quantifcation of starch by Titulation; the second method involved the colorful reaction with iodine using the UV equipment to measure the absorbance and calculate the percentage of starch; as a third method the FTIR was used, through which the concentration of the starch was calculated by the area under the curve obtained from the spectrum. Results. there is an efect of both the method and the raw material on the percentage of starch, while there was no efect of the interaction; the Tukey test indicates that the highest average percentage of extraction occurs with the extraction method by Titulation and with the starch of Manihot esculenta. Conclusion. It is used as raw material. Te method of quantifcation of starch by UV-VIS spectroscopy was the best for the study samples because it presented less deviation in relation to the FTIR and Titulation methods.