Author/Authors :
Franco Carneiro, William Department of Animal Science - Federal University of Lavras, Lavras, Minas Gerais, Brazil , Melo, Naiara Department of Animal Science - Federal University of Lavras, Lavras, Minas Gerais, Brazil , Duarte da Silva, Kiara Cândido Department of Veterinary Medicine - Federal University of Lavras, Lavras, Minas Gerais, Brazil , Simas Ferreira, Isabela Department of Animal Science - Federal University of Lavras, Lavras, Minas Gerais, Brazil , de Jesus Paula, Daniella Aparecida Department of Veterinary Medicine - Federal University of Lavras, Lavras, Minas Gerais, Brazil , Ferrante, Marcos Department of Animal Science - Federal University of Lavras, Lavras, Minas Gerais, Brazil , Solis Murgas, Luis David Department of Animal Science - Federal University of Lavras, Lavras, Minas Gerais, Brazil
Abstract :
The objective of this study was to determine the clove oil (CLO) dose as an anesthetic for tambacu juveniles (♂Piaractus mesopotamicus × ♀ Colossoma macropomum) at two temperatures. Sixty-four animals (2.42 ± 0.39 g
and 5.19 ± 7.5 cm) were used in a 4x2 factorial design, composed by four concentrations of CLO (25, 50, 75 and 100
mg L-1) and two temperatures (25 and 30 °C). Data were submitted to a two-way ANOVA followed by Tukey test.
Linear regression analysis was also applied to correlate the induction and recovery times of fish. Deep anesthesia was
achieved at all concentrations tested and no mortality was observed during the experimental procedure and after a
24-hour observation period. The shortest time for deep anesthesia (31.75 s) was observed at a concentration of 100
mg L-1 and a temperature of 30 °C, differing significantly from the other treatments. The longest recovery time (> 270
s) was observed at concentrations of 75 and 100 mg L-1 at both temperatures tested. A positive linear relationship
between induction time and deep anesthesia (P<0.05) and a negative linear relationship between total recovery time
(P<0.05) was observed. The CLO was effective to anesthetize tambacu juveniles in routine management activities. We
recommend to use the 50 mg L-1 concentration of CLO and a temperature of 30 °C when considering the induction
and recovery times for a deep anesthesia
Keywords :
Aquaculture , Neotropical fish , Management , Eugenol , Recovery
