Title of article :
Cryopreservation by Encapsulation-vitrification of Embryogenic Callus of Wild Crocus (Crocus hyemalis and Crocus moabiticus)
Author/Authors :
Baghdadi, S. H. Jordan University of Science and Technology - Faculty of Agriculture, Jordan , Shibli, R. A. University of Jordan - Faculty of Agriculture, Jordan , Syouf, M. Q. National Center for Agricultural Research and Extension(NCARE), Jordan , Shatanawi, M. A. Al-Balqa’a Applied University - College of Agriculture, Jordan , Arabiat, A. National Center for Agricultural Research and Extension(NCARE), Jordan , Makhadmeh, I. M. Jordan University of Science and Technology - Faculty of Agriculture, Jordan
Abstract :
Crocus is asexually propagated plant by corms and it is susceptible to a wide range of pathogens and environmental stresses. It cannot be effectively stored for long periods using conventional methods. Cryopreservation by encapsulation-vitrification was studied as a long-term conservation method. Callus fragments were encapsulated in 4 mm calcium alginate beads containing hormone-free MS medium. Encapsulated beads were dehydrated with the Plant Vitrification Solution 2 (PVS2) [(w/v) 30% glycerol, 15% DMSO and 15% EG] for 10, 20, 30, 60 and 90 minutes and dipped in liquid nitrogen for at least 1 h. Dehydration of cryopreserved encapsulated callus fragments with PVS2 for 20 minutes resulted in 100% survival and regrowth prior to cryopreservation for both species. After cryopreservation, the highest survival (55.6% and 75.0% for C. moabiticus and C. hyemalis, respectively) and highest regrowth (66.7% for both species) were obtained for 20 minutes dehydration with PVS2
Keywords :
Crocus hymalis , Crocus moabiticus , Encapsulation , Verification , PVS2
Journal title :
Jordan Journal of Agricultural Sciences
Journal title :
Jordan Journal of Agricultural Sciences
