Author/Authors :
Vagnini, Laura D. Paulista Center for Diagnosis Research and Training, Brazil , Nascimento, Adriana M. Paulista Center for Diagnosis Research and Training, Brazil , Canas, Maria do Carmo T. Paulista Center for Diagnosis Research and Training, Brazil , Renzi, Adriana Paulista Center for Diagnosis Research and Training, Brazil , Oliveira-Pelegrin, Gabriela R. Paulista Center for Diagnosis Research and Training, Brazil , Petersen, Claudia G. Paulista Center for Diagnosis Research and Training, Brazil , Petersen, Claudia G. Center for Human Reproduction Prof. Franco Jr., Brazil , Mauri, Ana L. Paulista Center for Diagnosis Research and Training, Brazil , Mauri, Ana L. Center for Human Reproduction Prof. Franco Jr., Brazil , Oliveira, João Batista A. Paulista Center for Diagnosis Research and Training, Brazil , Oliveira, João Batista A. Center for Human Reproduction Prof. Franco Jr., Brazil , Baruffi, Ricardo L.R. Paulista Center for Diagnosis Research and Training, Brazil , Baruffi, Ricardo L.R. Center for Human Reproduction Prof. Franco Jr., Brazil , Cavagna, Mario Center for Human Reproduction Prof. Franco Jr., Brazil , Franco, José G. Jr. Center for Human Reproduction Prof. Franco Jr., Brazil , Franco, José G. Jr. Paulista Center for Diagnosis Research and Training, Brazil
Abstract :
Objective: The aim of this study was to investigate the relationship between herpesvirus-associated ubiquitin-specific protease ( HAUSP A/G, rs1529916), tumor protein p53 ( TP53 Arg/Pro, rs1042522), leukemia inhibitory factor ( LIF G/T, rs929271), glycoprotein 130 ( gp130 A/T, rs1900173) and vascular endothelial growth factor ( VEGF G/A, rs1570360) polymorphisms and recurrent implantation failure (RIF) in Brazilian women. Subjects and Methods: A total of 120 women with RIF (i.e. those with ≥ 5 cleaved embryos transferred and a minimum of 2 failed in vitro fertilization/intracytoplasmic sperm injection attempts) were included. The control group involved 89 women who had experienced at least 1 live birth (without any infertility treatment). DNA was extracted from the peripheral blood of all participants, and the abovementioned single-nucleotide polymorphisms (SNPs) were genotyped by real-time polymerase chain reaction. The data were evaluated using Fisher’s test. Results: A significant difference between the RIF and control groups was found in the VEGF gene where the GG genotype showed a 2.1-fold increased chance of not being included in the RIF group, while the presence of an A allele increased this risk 1.6-fold. No significant differences were found for the other polymorphisms. Conclusion: This study showed an association between the VEGF –1154G/A polymorphism and RIF in Brazilian women.
