Title of article :
Preparation and Application of Grafted Fe(III) Protoporphyrin (IX) / Modified Nanoporous Silica as a Biocatalyst for Oxidative Reactions
Author/Authors :
Adhami, F. islamic azad university - Chemistry Department, ايران , Nazari, Kh. islamic azad university - Chemistry Department, ايران , Nazari, Kh. Research Institute of Petroleum Industry, N.I.O.C., ايران , Shahvelayati, A. islamic azad university - Chemistry Department, ايران
Abstract :
MCM-41 and amine-modified MCM-41 were synthesized. Then nanobiocatalysts were prepared through three different methods to graft hemin onto the modified nanosilicas of MCM-41 via the formation of the amide bonds. These biocatalysts were characterized by Atomic Absortion, IR, XRD, diffuse reflectance UV/Vis spectroscopies. These biocatalysts operated as a peroxidase enzyme model. They were compared to each other by oxidation of ABTS in the presence of hydrogen peroxide and tert-butyl peroxide. Kinetic parameters consist of initial rates, rate constant, Vmax, Km, Kcat, catalytic efficiency and turn over number were determined for the biocatalyst.
Keywords :
Modified MCM , 41 , peroxidase enzyme model , catalyticproperties.
Journal title :
Quarterly Journal of Applied Chemical Research (JACR)
Journal title :
Quarterly Journal of Applied Chemical Research (JACR)
