Effect of L-Carnitine and/or Sitagliptin on Serum Lipids Profile of H2 O2 Treated Rats (Part-1)

This study was designed to evaluate the ameliorative role of L-carnitine and/or sitagliptin on dyslipidemia induced by oral administration of 0.75% hydrogen peroxide in male rats. Thirty five (35) adult male rats were randomly and equally divided into five groups (C, T1, T2, T3 and T4) and were treated for 60 days as following: Group C (Control group), rats in all treated groups from T1 to T4 were given 0.75% H2 O2 in drinking water. In addition to H2 O2 , 100mg/kg B.W of L-carnitine and 1.5mg/kg. B.W of sitagliptin were administered orally to rats in groups T2 and T3 respectively, while rats in group (T4) were administered orally. Combination of sitagliptin (0.75 mg/kg. B.W) plus L-carnitine (50mg/kg B.W.) Fasting (8-12 hrs.) blood samples were collected by Retro-orbital sinus technique at different times 0, 30 and 60 days of the experiment and serum were collected for measuring the following parameters: serum lipid profile including: total cholesterol (TC), triacylglycerol (TAG), cholesterol in High density Lipoprotein (HDL-c), Low density lipoprotein (LDL-c) and very low density lipoprotein (VLDL-c) concentrations. The results showed a case of dyslipidemia in rats exposed to H2 O2 manifested by significant (p<0.05) increase in serum TC, TAG, LDL-C and VLDL-C with significant (p<0.05) decrease in serum HDL-C concentrations comparing to other treated groups. The protective (hypolipidemic) role of L-carnitine and sitagliptin was clarified in groups (T2, T3) manifested by hypocholesterolemia. It exhibited significant (p<0.05) decrease in serum, TAG, LDL-c, and VLDL-c concentration with significant (P<0.05) elevation in serum HDL-c concentration.