Author/Authors :
Manoj, D. Indian Institute of Food Processing Technology - Thanjavur - 613005 Tamil, India , Auddy, Ishita Indian Institute of Food Processing Technology - Thanjavur - 613005 Tamil, India , Nimbkar, Shubham Indian Institute of Food Processing Technology - Thanjavur - 613005 Tamil, India , Chittibabu, S. Indian Institute of Food Processing Technology - Thanjavur - 613005 Tamil, India , Shanmugasundaram, S. Indian Institute of Food Processing Technology - Thanjavur - 613005 Tamil, India
Abstract :
The screen-printed electrode biosensor was developed for triglyceride determination in coconut milk. The biosensor was developed
by adding lipase, glycerol-3-phosphate (GPO), and glycerol kinase (GK), which is immobilized to a gelatin solution. The
concentration of triglyceride is found to be linear to the current produced. The developed screen-printed electrode biosensor
showed the optimum response for pH 7.0, 45 mg amount of gelatin, 2.5% glutaraldehyde concentration solution. The developed
biosensor was able to find triolein concentrations 0.1 to 1.5 mM. The correlation obtained between these two methods was 93% which was found to be good.
Keywords :
Development , Screen-Printed Electrode Biosensor , Rapid Determination , Triglyceride Content , Coconut Milk
