Title of article :
Genetic Diversity among Different Samples of Camel’s Tick (Hyalomma dromedarii) in Taif city, Saudi Arabia
Author/Authors :
Albogami, Bander Department of Biology - College of Sciences - Taif University, Saudi Arabia
Abstract :
The ticks (Hyalomma dromedarii) are significant disease transmit vectors to camel in Saudi Arabia. They
transfer a range of pathogens such as bacteria, protozoa and viruses to people and animals. Their significance as a
malady vector requires dependable molecular technique in the present research, the genetic variability of H. dromedarii
from six farms located in Taif governorate of Saudi Arabia were investigated by using ISSR marker technique. Eight
ISSR markers were chosen to deliver clear and reproducible polymorphic loci. The genetic variability within and
among all samples of H. dromedarii were investigated via these markers. A total of 102 DNA loci were produced. Out
of these loci, 63 were with ratio of 61.8%. The number of total bands varied from 8 to 16 bands, and the bands size
ranging from 120 to 2100 bp. By using Neighbor-joining genetic distance method, the 12 samples were assembled into
two main group with 0.56 genetic similarity. The first group contains two samples, number 2 and 11, from different
farm. The other ten samples are located in second group which divided into two clusters. The value of genetic similarity
varied from 0.877 for two samples, tick-9 and tick-12 to a lowest value of 0.056 for samples tick-3 and tick-11. The
present data indicates that there is no remarkable correlation between the geographic distance and genetic similarity
Keywords :
Genetic diversity , Hyalomma dromedarii , ISSR-PCR , Taif city , Saudi Arabia
Journal title :
Advances in Animal and Veterinary Sciences