Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from Döner kebab sold in Dramaga Bogor, West Java Province, Indonesia. A total of 43 samples of Dὂner kebab meat were taken from all of the vendors in the radius of 2 km around the border of IPB University campus, Dramaga Bogor. Isolation and identification of E. coli were referred to the Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk in SNI 2897:2008 issued by the National Standardization Agency of Indonesia. The resistance against nine antibiotics were tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that nine samples were positive containing E. coli (20.9%) and 84.2% of E. coli isolates were resistant to eight antibiotics: gentamicin (57.9%), ampicillin (26.3%), cefotaxime, trimethoprim-sulfamethoxazole, and ciprofloxacin (21.1%), amoxicillin-clavulanate (10.5%), enrofloxacin, and oxytetracycline (5.3%). All of E. coli isolates were susceptible to chloramphenicol (100%). There were 21.1% of isolates also resistant to three or more classes of antibiotics that were known as multi-drug resistant (MDR) with different resistant patterns.The presence of E. coli resistant antibiotic in kebab meat could results serious problems in human health.