Author/Authors :
Dileepkumar, Kodakkadan Manikkan Department of Veterinary Surgery & Radiology - College of Veterinary and Animal Sciences - Mannuthy - Thrissur - Kerala, India , Kumar Maiti, Swapan Surgery Division - Indian Veterinary Research Institute - Izatnagar - Uttar-Pradesh, India , Naveen, Kumar Surgery Division - Indian Veterinary Research Institute - Izatnagar - Uttar-Pradesh, India , Ravindran Ninu, Ajantha Department of Veterinary Surgery & Radiology - Veterinary College & Research Institute - Tirunelveli - TANUVAS - Tamil Nadu, India , Mitra, Abhijit Surgery Division - Indian Veterinary Research Institute - Izatnagar - Uttar-Pradesh, India
Abstract :
Mammary neoplasms are the most frequently observed neoplasms in female dogs and constitute an important problem in veterinary medicine. The objective of this study was to investigate the p53 and Cox-2 gene expressions
profile in mammary tumours of dogs of different histological types in comparison to healthy mammary tissues using
quantitative real time PCR (qPCR) assay. A total of 12 cases (5 benign lesions: adenomas and benign mixed tumour
and 7 malignant lesions: carcinomas and malign mixed tumour) were examined. Higher expression levels for p53 and
Cox-2 genes in mammary tumour tissues were observed as compared to normal mammary tissues. Expression of both
genes was relatively higher in malignant tumour than benign. These results show that p53 and Cox-2 genes may be
useful tumour markers for early evaluating malignancy in canine mammary tumours.
Keywords :
Dog , mammary tumours , p53 , Cox-2 , tumour markers
