Effect of Lactobacillus acidophilus on some Physiological and Immunological Aspects in Experimentally Induced Colitis in Rats

Inflammatory bowel diseases of two forms, Ulcerative colitis and Cron’s disaes cause inflammation and ulcers in the colon resulting in disruption of normal colonic function and altered physiological aspects. The present study aimed to investigate the beneficial effects of Lactobacillus acidophilus (LBA) as a probiotics in reducing the severity of this disease. Forty eight male rats were distributed into four groups, each of 12. 1st consider as control (C), 2nd and 4th (T1&T3) have colitis, 3rd and 4th (T2 &T3) administered Lactobacillus acidophilus in a dosage of (5 × 108 CFU). Colitis induced experimentally by intrarectal infusion of acetic acid. Food intake, body weight gain and food conversion ratio (FCR) recorded for 5th and 25 the days of colitis Blood samples were collected from experimental animals at the days 1, 5 & 25 of experiment. Food intake and bodyweight gain were reduced in treated animals but FCR was improved by the administration of lactobacillus acidophilus improved FCR in spite of reduction of food intake and bodyweight gain. Total protein and globulins were increased by L. Acidophilus in normal and acetic acid colitis rats. Total WBCs lymphocytes, and platelets showed a gradual increase by L. acidophilus while neutrophils increased in acetic acid induced colitis. Immunologically, Leptin increased sharply during the 1st in colitis followed by then followed by gradually decrease gradual decrease while in increased. Il-6 increased sharply in colitis and lactobasillus during 1st day Administration of L. Acidophilus results in beneficial effects on the alteration of some physiological measurements of acetic acid induced colitis and make balance between proinflammatory and anti-inflammatory response.