Author/Authors :
Yamanappa Mukartal, Shivalingappa Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Rathnamma, Doddamane Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Narayanaswamy, Hogalagere Doddappaiah Department of Pathology - Veterinary College, Hebbal - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Isloor, Shrikrishna Department of Veterinary Microbiology - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Singh, Shoorvir Division of Animal Health - Central Institute for Research on Goats (CIRG) - Makhdoom - PO-Farah - Uttar Pradesh, India , Marinaik Chandranaik, Basavegowdanadoddi Institute of Animal Health and Veterinary Biologicals (IAH&VB) - Hebbal , Bengaluru , Methuku, Shobha Rani Institute of Animal Health and Veterinary Biologicals (IAH&VB) - Hebbal , Bengaluru , Elattuvalappil, Anuradha Menon Department of Pathology - Veterinary College, Hebbal - Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Bengaluru , Mallaiah, Srikanth Institute of Animal Health and Veterinary Biologicals (IAH&VB) - Hebbal , Bengaluru , Shambanna, Manjunath Suranagi Livestock Research and Information Centre (Sheep) Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU) - Nagamangala, India
Abstract :
Ovine Johne’s disease (OJD) is a chronic incurable inflammation of intestines caused by Mycobacterium
avium subspecies paratuberculosis (MAP). Prevalence of OJD was estimated in the Bannur breed of sheep located in
Mandya district of Karnataka state on the basis of clinical signs and detecting presence of bacilli in faeces and antibodies in serum of naturally infected sheep. Clinical samples (faeces-135, blood-45 and serum-100) were collected
from sheep located at LRIC (Livestock Research and Information Centre) farm and Dangur sheep breeding farm.
Sheep at LRIC farm (>15.0%) exhibited clinical symptoms of sub-mandibular oedema, emaciation, alopecia and were
suspected for JD. None of the sheep at Dangur farm, had clinical symptoms of JD. Of 168 samples (LRIC farm), 30
(60.0%), 38 (76.0%), and 24 (35.2%) were positive by microscopy, ELISA and IS900 PCR, respectively. Whereas of
112 samples (Dangur farm), 5 (10.0%), 7 (14.0%) and 6 (50.0%) were positive by microscopy, ELISA and IS900 PCR
respectively. Overall prevalence of JD was 54.7 and 16.0% in LRIC and Dangur farms, respectively. Microscopy and
ELISA were screening tests and PCR was confirmatory. Prevalence of OJD was moderately high in Bannur breed of
sheep and combinations of microscopy and ELISA followed by PCR may be adopted as a strategy for screening and
diagnosis of JD in sheep.
Keywords :
Johne’s disease , Mycobacterium avium subspecies paratuberculosis , Microscopy , ELISA , IS900 PCR
