Author/Authors :
Widjiati Post Graduate School of Universitas Airlangga Surabaya, Indonesia , Faizah, Zakiyatul Departement of Biomedical Science Faculty of Medicine Universitas Airlangga Surabaya, Indonesia , Darsini, Ninik Departement of Biomedical Science Faculty of Medicine Universitas Airlangga Surabaya, Indonesia , Fitri Hendrawan, Viski Departement of Reproduction - Faculty of Veterinary Medicine - Universitas Brawijaya Malang, Indonesia , Luqman, Epy Muhammad Departement of Veterinary Anatomy - Faculty of Veterinary Medicine - Universitas Airlangga Surabaya, Indonesia , Sumitro, Sutiman B. Departement of Biology Faculty of Science Universitas Brawijaya, Malang, Campus B Universitas Airlangga, Jalan Airlangga, Surabaya
Abstract :
The weakness of in vitro fertilization (IVF) was not able to observe whether in vitro fertilized oocytes
were all mature. Intra Citoplasmic Sperm Injection (ICSI) was able to solve IVF failure as spermatozoa was directly
injected to oocyte. The research was divided into 2 groups, T1 group: oocyte was fertilized conventionally (maturated
oocyte was supplamanted with spermatozoa) and T2 group: fertilization using ICSI method. Next, oocyte was mixed
with spermatozoa. After ICSI was done, it was put into incubator CO2
5 %, with the temperature of 38.5o
C. Then,
fertilization rate and embryo growth at cleavage stage were observed. The data presented as means Standard Error of
the Mean (S.E.M) and were compared using the Student’s t-test. Research result showed that fertilization rate of ICSI
group was higher significant than conventional IVF group (p<0.05). Also, cleavage stage (2 cells, 4 cells, 8 cells) of ICSI
group was higher significant than conventional IVF group (p<0.05). The research concluded that fertilization rate and
embryo cleavage of IVF using ICSI technique were higher than conventional IVF.
Keywords :
Sperm injection , Oocytes , Cell block , Fertilization rate , Embryo growth
