Author/Authors :
Ratta, Barkha Division of Biochemistry - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India , Priyadarsini, Swagatika Division of Biochemistry - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India , Channabasappa, Nikhil K. Division of Biochemistry - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India , Mani, Pashupathi Division of Biochemistry - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India , Saxena, Meeta Division of Biochemistry - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India , Saravanan, R Immunology Section - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India , Kumar, Ajay Division of Biochemistry - Indian Veterinary Research Institute, Izatnagar, Bareilly (UP), India
Abstract :
Bovine herpesvirus 1 (BoHV-1) affects mainly large ruminants (cattle and buffalo), resulting in a substantial
negative impact on the industry. The virus causes respiratory symptoms, abortion and a decrease in milk production.
Among envelope proteins of the virus, the glycoprotein D (gD) is considered a dominant immunogenic. We expressed
the immunogenic portion of gD in the prokaryotic system (E. cloni 10G) and did evaluate its potential as a diagnostic
antigen for indirect ELISA. Using the Ni-HRP probe, the expressed recombinant gD protein (rgD, 45kDa) was
characterized by SDS-PAGE and western blotting. The immunogenic reactivity of red was optimized for indirect
ELISA using known true positive and negative sera (antibodies) of the virus. Sera (n=158) were screened and, with
a cut-off value of 0.238, relative diagnostic sensitivity (69%) and specificity (52.3%) of in-house developed indirect
ELISA was determined. With these preliminary results, the study suggests the diagnostic potential of recombinant gD
protein for serodiagnosis of BoHV-1 infection at a large scale.
Keywords :
Indirect ELISA , Bovine herpesvirus-1 , Glycoprotein D , Antibodies , Recombinant
