Author/Authors :
Widyaningrum, Yeni Indonesian Beef Cattle Research Station, Pasuruan, Indonesia , Luthfi, M. Indonesian Beef Cattle Research Station, Pasuruan, Indonesia , Ratnawati, Dian Indonesian Beef Cattle Research Station, Pasuruan, Indonesia , Affandhy, Lukman Indonesian Beef Cattle Research Station, Pasuruan, Indonesia , Aulanni’am Department of Biology - Faculty of Mathematics and Natural Sciences - University of Brawijaya, Malang, Indonesia , Warih Marhendra, Agung Pramana Department of Biology - Faculty of Mathematics and Natural Sciences - University of Brawijaya, Malang, Indonesia , Tribudi, Yuli Arif Faculty of Agriculture - Universitas Tanjungpura, Pontianak, Indonesia
Abstract :
Pregnancy-specific protein B (PSPB) is produced by binucleate trophoblast cells in the bovine placenta
around the time of implantation. We produced anti-PSPB polyclonal antibodies to detect PSPB in the serum of
Peranakan Ongole (PO) pregnant cows. Six New Zealand White (NZW) Rabbits were immunized with 40-60 kDa
protein in combination with adjuvants for antibody production. Sera of experimental animals (n = 30) were taken
from the auricular vein and subjected to the ELISA test. The highest antibody titer was used to test the specificity of
antibodies using the dot blot method. Antibody titer was observed during the first week, while the highest antibody
titer (OD = 0.3755) was found during the 3rd week of bleeding, which decreased gradually during the subsequent
weeks. The sensitivity test showed an ability of anti-PSPB to detect PSPB in the serum of pregnant cows from 90 days
of gestation and as low as 1:120. The study concludes the potential utility of anti-PSPB polyclonal antibodies for the
detection of pregnancy in cows.
Keywords :
Cattle , Anti-PSPB , Polyclonal antibodies , Diagnosis of pregnancy
