GC-MS Evaluation of Essential Oil Constituents fromRosaDamascenaWild Rose:Effect of Season and ClimaticConditions

The presentstudy describes theextraction and identification of essential oil constituents fromRosadamascenawild rose as well as effect of seasonand climatic conditions of selected regions (KPK,Pakistan). Essential oil constituents were extracted using Steam distillation (SD) method, whereasthe analysis of the extracted components were carried out using GC coupled with MS. A total ofseven oil components namely; o-cymene, d-limonene, (R)-(+)-citronellal,n-heneicosane, eugenolmethyl ether, p-menth-1-en-8-ol and eucalyptol were extracted and quantified. GC-MS analysisindicated that the Rosa Damascena Mill contains several important volatile constituents i.e. (R)-(+)-citronellal, o-cymene, d-limonene, eucalyptol, p-menth-1-en-8-ol, eugenol methyl ether and n-heneicosane with extraction yield 3.8% to 91.9%. Seasonal effect investigation indicated thatspring season showed high essential oil yield as compare to summer and winter. The chemicalcomposition of volatile components analyzed in the essential oil, extracted from rose petalscollected from Bannu and Peshawar in spring indicates high concentration of all components inBannu petals as compared to Peshawar. Present research indicated that the geographicaldistribution and seasonal variation highly affect the essential oil constituents inRosa damascenawild rose.