Capillary Gas Chromatographic Determination of GammaAminobutyric acid and Putrescine in Cerebrospinal Fluidusing Trifluoroacetylacetone as Derivatizing Reagent

In present work, a new capillary gas chromatographic procedure was established and validated forthe determination of gamma aminobutyric acid (GABA) and putrescine (Put) usingtrifluoroacetylacetone (FAA) as derivatizing reagent from Cerebrospinal Fluid (CSF) samplesprior to their gas chromatographic-flame ionizationdetector (GC-FID) analysis. GABA, Put,cadaverine (Cad) and tyramine (TY) as imitative of FAA extracted from the column HP-5 (30 m x0.32 mm i.d) at temperature 110ºC for 1 min, tracked by heating rate 25ºC to 260ºC /min. Thedetection was carried outby FID with segregate ratio 10:1,v/vwith whole run time 10 min.Theproposed method showedlinear calibrationrange between2.5-50 μg/mL withlow limit ofdetection1.0-2.5 μg/mL analogous to 0.1 ng to 0.25 ng for selected Put, Cad, GABA, and TY.The method based on the pre-concentration was used for the determination of GABA and Putfrom CSF of human being and amounts found were 0.25-0.56 μg/mL and 0.16-0.41 μg/mL withrelative standard deviation (RSD) within 0.8-1.1 and 1.1-1.5 %, respectively.Many of amino-acids tested, separated completely and did not variate the determinations of GABA and Put.